International Standard
ISO 13885-3:2020
Gel permeation chromatography (GPC) — Part 3: Water as eluent
Reference number
ISO 13885-3:2020
Edición 1
2020-07
International Standard
Vista previa
ISO 13885-3:2020
77485
No disponible en español
Publicado (Edición 1, 2020)

ISO 13885-3:2020

ISO 13885-3:2020
77485
Idioma
Formato
CHF 129
Convertir Franco suizo (CHF) a tu moneda

Resumen

This document specifies the determination of the molar-mass distribution and the average molar mass values Mn (number average) and Mw (weight average) of polymers that are soluble in water by gel permeation chromatography (GPC).

NOTE Also known as size exclusion chromatography (SEC).

This method is applicable to neutral polymers and polyanions (e.g. polycarboxylates, polysaccharides, fully hydrolyzed polyvinyl alcohols and high-molecular polyethylene oxides). It is not applicable to polycations [e.g. polyvinylpyrrolidone, polyvinylpyridine, salts of poly(diallyl‑N,N‑dimethyl‑azacyclopentane), chitosan].

Despite good solubility in the mobile phase and even though the chromatograms obtained show good repeatability, it is possible that this method cannot be used with certain polymer types because of specific interactions (e.g. adsorption) within the sample/eluent/column system (see also Clause 12).

The conditions specified in this document are not applicable to the GPC analysis of polymer samples with Mw values greater than 106 g/mol and/or polymers with elution limits outside the calibration range (see 7.6 and Annex C).

This document includes no correction methods (e.g. for the elimination of peak broadening). If absolute molar mass values are required, an absolute method (e.g. membrane osmometry for Mn or light scattering for Mw) can be used.

Informaciones generales

  •  : Publicado
     : 2020-07
    : Norma Internacional publicada [60.60]
  •  : 1
     : 23
  • ISO/TC 35/SC 16
    87.060.20 
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